Polymerase Chain Reaction

Techniques used for replication of a single strand of DNA to provide sufficient quantity for other applications.

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Attotron Biosensor Corporation
Research and development company for development of biosensors and related products for the research and educational markets.
BioRad, Amplification, PCR
Division of BioRad Laboratories that manufactures and sells instruments for PCR, in Hercules, California, USA.
GeneOhm Sciences
Tests on group B Streptococcus and methicillin resistant Staphylococcus aureus by PCR / DNA sequencing.
Manufacturer of material for differential display PCR in Nashville, Tenn USA.
HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
Ingenetix GmbH
Develops technology and products for DNA and mRNA research. Also provide DNA testing for the determination of parentage/paternity and custom DNA sequencing, oligonucleotide synthesis, genotyping services, pharmacogenetics and quantitative PCR, in Vienna, Austria.
Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation
Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
Inverse PCR for Use with Snyder mTn-lacZ/LEU2-based Mutagenesis
Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.
Inverse PCR Protocol
Step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.
Kary B. Mullis - Autobiography
The originator of PCR, from the Nobel e-museum web site.
Nematode ITS1 Size Variation
Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes, from University of Nebrasca.
PCR Amplification of cDNA Segments by 2 Stage Nested PCR
Protocol from the method database of NIH, USA.
PCR Animated
Animation illustrating the principle of PCR, from the University of Ghent, Belgium.
The PCR Encyclopedia
Describes plans for a free encyclopedia dedicated to the polymerase chain reaction (PCR).
PCR Gateway
A directory of PCR techniques, PCR protocols, PCR troubleshooting, PCR websites and online resources from the publisher Horizon Press.
PCR Guru
A downloadable textbook on PCR setup and optimization, not free.
PCR Protocol
Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.
PCR Protocols
Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University
PCR-ELISA and Related
P Zhang, CJ Gebhart, D Burden, GE Duhamel: A low technology alternative to real time PCR, technical article on the site of BT&C, Inc Bridgewater, NJ, USA.
International biotechnology company specialized in a design service for PCR primers and probes, PCR-based technology development, projects for development of polymorphism and software development.
Free online tool for generation of PCR primers.
Principle of PCR
Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.
Protocols Online: PCR Protocols
Extensive collection of PCR protocols and methods from Protocol On Line.
Rational Primer Design Greatly Improves Differential Display-PCR (DD-PCR)
Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.
Reference in PCR
Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.
Roe Laboratory Protocols
Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.
Single Tube Confirmation PCR Protocol
For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.
T-DNA Generated Enhancer Traps in Arabidopsis
Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH.
Thermostable DNA Polymerases
Discussion of their origin and briefly their properties. From the web site of Colorado State University.
The Web Guide of PCR
List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.
Which DNA Marker for Which Purpose?
Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Göttingen.

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October 16, 2016 at 23:03:09 UTC
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